Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid
نویسندگان
چکیده
منابع مشابه
Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...
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background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...
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Background: Many sperm characteristics including, motility and morphology are important for normal spermatozoa- oocyte intraction. These features may be altered during vitrification. Motile sperm organelle morphology examination (MSOME) is an unstained real time high magnification technique that analysis viable sperm morphology instantly. This study aimed to investigate the influence of vitrifi...
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The aim of this study was to develop and to test the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or in vitro fertilization [IVF]). To test the effect of vitrification on basic sperm parameters, each of 68 swim-up-prepared ejaculates from oligo-astheno-terato-zoospermic patien...
متن کاملCreatine Kinase Activity in Human Seminal Fluid
Introduction: Male infertility is an important factor of infertility in couples. In most cases it is caused by defects in sperm cell function; whether low sperm cell count or reduced motility along with altered morphology. Defects in sperm cells development are influencing enzyme activities in the seminal fluid. Aim: This research tried to determine can activity of creatine kinase (CK) in semin...
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ژورنال
عنوان ژورنال: Andrology
سال: 2016
ISSN: 2047-2919
DOI: 10.1111/andr.12212